1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEFerrostatin-1Cat. No.: HY-100579CAS No.: 347174-05-4分式: CHNO分量: 262.35作靶點(diǎn): Ferroptosis作通路: Apoptosis儲(chǔ)存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect fromlight)溶解性數(shù)據(jù)體外實(shí)驗(yàn) DMSO : 150 mg/mL (571.76 mM

2、)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制備儲(chǔ)備液1 mM 3.8117 mL 19.0585 mL 38.1170 mL5 mM 0.7623 mL 3.8117 mL 7.6234 mL10 mM 0.3812 mL 1.9059 mL 3.8117 mL請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲(chǔ)備液，并請(qǐng)注意儲(chǔ)備液的保存式和期限。體內(nèi)實(shí)驗(yàn) 請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥式選擇適當(dāng)?shù)娜芙獍福渲魄罢?qǐng)先配制澄清的儲(chǔ)備液，再依次添加助溶劑(為保證實(shí)驗(yàn)結(jié)果的可靠性，體內(nèi)

3、實(shí)驗(yàn)的作液，建議您現(xiàn)現(xiàn)配，當(dāng)天使；澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；以下溶劑前的百分 指該溶劑在您配制終溶液中的體積占)：1. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (9.53 mM); Clear solution2. 請(qǐng)依序添加每種溶劑： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (9.53 mM); Clear solutionBIOLOGICAL ACTIVITY1/2 Master of Small Molecules 您邊的抑制劑師ww

4、w.MedChemE物活性 Ferrostatin-1是ferroptosis 的強(qiáng)效抑制劑，其 EC50 值為 60 nM。IC50 & Target EC50: 60 nM (Ferroptosis) 1體外研究 Ferrostatin-1 is the most potent inhibitor of erastin-induced ferroptosis in HT-1080 cells (EC50=60 nM).Ferrostatin-1 does not inhibit ERK phosphorylation or arrest the proliferation of HT-10

5、80 cells. Ferrostatin-1does, however, prevent erastin-induced accumulation of cytosolic and lipid ROS 1. Cells pretreated with 0.4 M Ferrostatin-1 displays significantly reduce intracellular reactive oxygen species (ROS) and nitrogen species(RNS) below basal levels. Additionally, increased intracell

6、ular ROS levels are also significantly lowered belowbasal levels by a 0.4 M Ferrostatin-1 pretreatment. Ferrostatin-1 treatment for 24 h does not change theexpression level of i-NOS in SHSY-5Y cell when compare with vehicle (0.02 % DMSO) treated cells 2.PROTOCOLKinase Assay 2 The cells treated for 2

7、4 h with Ferrostatin-1 are washed three times with phosphate-buffered saline (PBS)and pelleted by centrifugation. The supernatant is removed and 80 L of lysis buffer is added to the cells andthen stored overnight at -20C. Subsequently, the cells are centrifuged at 10,000 RPM for 12 min and boththe p

8、ellet and supernatant are stored for future use 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Cell Assay 2 SH-S5Y cells are seeded in 24-well plates. After overnight incubation, the cells are exposed for 24 h to 1 Mof Ferrostatin-1, under standard g

9、rowth conditions. Bright field photomicrographs are captured using aninverted microscope equipped with 10 objective in a live-cell modality 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產(chǎn)品發(fā)表的科研獻(xiàn) Cell Death Dis. 2019 Apr 15;10(5):331. Environ Poll

10、ut. 2019 Aug. Biomater Sci. 2019 Mar 26;7(4):1311-1322. Artif Cell Nanomed B. 2019 Dec;47(1):3087-3093. Drug Des Dev Ther. 2019 Jul.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Dixon SJ, et al. Ferroptosis: an iron-dependent form of nonapoptotic cell death. Cell. 2012 May 25;149(5):1060-72.2. Kabiraj P, et al. The neuroprotective role of ferrostatin-1 under rotenone-induced oxidative stress in dopaminergic neuroblastoma cells.Protein J. 2015 Oct;34(5):349-58.McePdfHeig t Caution: Product has not been f