1、免疫細胞表面抗原分子 CD家族對照表(CD1-CD247)Exalpha Biologicals produces and sells products for Life Science Research, including monoclonal and polyclonal antibodies, for use in immunohistochemistry, western blot, ELISA and Flow Cytometry. We offer over 800 products and, through collaborations with other scientist

2、s as well as our own in-house R&D programs, Exalpha continues to bring leading edge products to market. Our family of CD antibodies combines quality and performance with reasonable prices to make our CD antibodies the best value in the market today. Our family of Proliferation products offer easy al

3、l-in-one kits or stand alone antibodies to quantitate proliferating cells. Take a look at our website or call us for additional information.Note: no information is available on CDw145, CD181, CD182, CD185-194, CD196-199, CD211, CD214-216, CD218-220, CD237Backgro undCD: cluster designation of monoclo

4、nal antibodies (clusters of differentiation)Desig nated at the 1st to 7 th Workshops on Intern ati onal Huma n LeukocyteDifferen tiati on An tige nsThe last con fere nee was in 2000. The n ext con fere nee is in 2004.HLDA Workshops are the primary mecha nism to characterize leukocyte surfacean tige

5、nic molecules and epies; erythroid an tige ns are now in cludedFor 1st to 6th workshops, an tibodies were submitted to the orga nizing laboratory,coded and sent to participati ng laboratories for testi ng aga instvarious cell types. Forthe 7th workshop, a CD designation could be established for a mo

6、lecule if its genehas been cloned and at least one specific monoclonal antibody hadbeen studied in theWorkshopInterpretation should be based on cellular distribution of staining, proportion ofpositively stained cells, staining intensity and cutoff levels.CD1Family of non-polymorphic MHC class I-like

7、 glycoprote insAlso member of immu no globulin superfamilyOn chromosome 1q22-23 (not MHC lin ked)Has 5 differe nt subsets, all non covale ntly associated with 12 kd beta 2 microglobuli nFun cti on:restrict T cell resp on ses to certa in an tige ns; may mediate thymic T celldevelopme ntPositive stai

8、ning (no rmal):cortical thymocytes (70%), activated T cells, Lan gerha ns cells,interdigitating dendritic cellsPositive sta ining (disease):pre T ALL with cortical thymocyte phe no type; Lan gerha ns cellhistiocytosisNegative stai ning:mature peripheral T cellsCD1aPositive sta ining (no rmal):Den dr

9、itic cells in dermis/epidermis of benign in flammatory skindisordersPositive stai ning (disease):Lan gerha ns cell histiocytosis (fairly specific), myeloid leukemias,some B cell malig nan cies; den dritic cells in most peripheral cuta neous T celllymphomas, AJCP 2001;116:72Negative stai ning:no rmal

10、 B cells, most cuta neous peripheral B cell lymphomas (? reflectsreplaceme nt of reactive patter n containing den dritic cells with a n eoplastic pattern of B cells)Micro images:Lan gerha ns cell histiocytosisMicro images (AJSP subscribers):pulm onary Lan gerha ns cell histiocytosisMicro images (Hum

11、 Path subscribers):Lan gerha ns cell histiocytosisRefere nces: AJSP 2001;25:630CD1bPositive stai ning (disease):myeloid leukemias and some B cell malig nan ciesNegative stai ning:no rmal B cellsCD1cPositive sta ining (no rmal):subset of no rmal peripheral B cellsPositive stai ning (disease):myeloid

12、leukemias and some B cell malig nan ciesNegative stai ning:no rmal B cellsCD1dPositive stai ning (no rmal): thymus (low levels), bowelCD1eCD2Aka E rosette receptor, LFA-2 (leukocyte fun cti on an tige n)Fun cti on:binds CD58 / LFA-3 on an tige n-prese nting cells, and in duces costimulatory sig nals

13、in T cellsAlso regulates T and NK-mediated cytolysis, inhibits apoptosis of activated peripheral Tcells, mediates T cell cytok ine producti on, regulates T cell an ergyPositive stai ning (no rmal):thymocytes (95%), mature peripheral T cells (almost all), NK cells(80-90%), thymic B cells (50%)Micro i

14、mages:extra no dal NK/T cell lymphoma, n asal typeCD2RCD2 epies restricted to activated T cellsPositive stai ning: activated T cells, ? NK cellsCD3Aka 0KT3Fun cti on: complex (5 cha ins) of i ntegral membra ne glycoprote ins assembled as a complex; has long cylasmic tail with antigen recognition act

15、ivation motif; complex is then down regulatedAlso subdivided into delta, epsil on, gamma subtypesCylasmic expressi on at early T cell differe ntiati on, the n membra nous expressi onMost specific T cell an tibodyPositive stai ning (no rmal):thymocytes, peripheral T cells, NK cells; also Purkinje cel

16、ls ofcerebellumPositive sta ining (disease):80% of T cell lymphomasNegative stai ning:gamma delta T cell receptors, most B cell lymphomasMicro images:CD3 epsilo n-testicular NK/T cell lymphoma (figure 3D)Micro images (AJSP subscribers):achalasia, post-tra nspla nt lymphoproliferativedisease in liver

17、Refere nces:AJSP 2001;25:1413CD4Aka 0KT4, T helper/ in ducerOn chromosome #12pNon polymorphous glycoprote ins bel onging to immu no globuli n superfamilyServes as HIV receptor on T cells (as do chemoki ne receptors CCR5 and CXCR4),macrophages, bra inCD4+ T cells are killed by HIVCoreceptor in MHC cl

18、ass II-restricted antigen induced T cell activationBinds to non polymorphic regi on of class I molecules; may in crease avidity of cell-cellin teracti onsPositive stai ning (no rmal):thymocytes (80-90%), mature T cells (65%, T helper andCD4/CD8+ thymocytes), macrophages, Lan gerha ns cells, den drit

19、ic cells, gran ulocytesPositive sta ining (disease):pityriasis liche no idesMicro images:acute demyeli nati ng disease, extra no dal NK/T cell lymphoma, n asal typeCD5Bel ongs to an cie nt scave nger receptor familyIs physically and functionally coupled with T cell receptor-zeta-CD3 signal transduce

20、r complexCD5+ B cells produce“ ge neralist an tibodies” - polyreactive low affinity“ n aturalan tibodies to exoge nous an tige ns (teta nus toxoid, lipopolysaccharide) as well as autoreactive an tibodies (ssDNA, thyroglobuli n, in suli n)Note: sharks only have polyreactive IgMNote: mono reactive IgG

21、 is produced by 0.1% of circulati ng B cells, from positive selecti on and somatic point mutati onFirst line of defe nse aga in st an tige ns; have a low activati on threshold; are the on ly line of defe nse for those who cannot produce specific an tibodyProduce antibodies using germ line (non mutat

22、ed) configuration of gene segments, usually IgMProduction elevated in rheumatoid arthritis (27-52% of circulating B cells vs. 20%no rmal)CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signalsdepe nding both on the cell type and the developme nt stagePositive sta ining (no

23、rmal):B cells of man tle zone of splee n and lymph no des; B cells inperitoneal and pleural cavities; almost all T cells;In fetus, most B cells in splee n and cord blood are CD5 positivePositive sta ining (disease):B cell CLL/SLL, man tle cell lymphoma, most T malig nan cies,thymic carc ino mas (70%

24、)Negative stai ning:spin dle cell thymomas, MALT lymphoma, follicular lymphomaMicro images:extra no dal NK/T cell lymphoma, n asal type, man tle cell lymphoma (figure3D)CD6Adhesion molecule mediating the binding of developing thymocytes with thymicepithelial cellsMay be in volved in autoimmu nity an

25、d graft vs. host disease (GVHD)An tibodies to CD6 are used to deplete T cells from bone marrow tra nspla nts to preve ntGVHDPositive stai ning (no rmal):low levels on immature thymocytes, high levels on maturethymocytesCD7Membra ne glycoprote in and Fc receptor for IgMHomologous to TCR gamma, Ig kap

26、paMembra ne expressi on early duri ng T on toge ny, before TCR rearra ngeme nt, persistsun til term inal stages of T cell developme ntLower expressi on in memory T cells vs. n aive T cellsPositive stai ning (no rmal):mature peripheral T cells (85%), post-thymic T cells(majority), NK cells (majority)

27、, some myeloid cellsPositive sta ining (disease):T cell ALL; AML (especially M4/M5), chr onic myeloge nousleukemia, blasts in transient myeloproliferative disorderNegative expressi on:B cell ALL, Sezary syn drome, adult T cell leukemia/lymphomaMicro images:extra no dal NK/T cell lymphoma, n asal typ

28、eCD8Aka OKT8, T cell suppressor/cytotoxic cellsOn chromosome #2MHC class I restricted receptor; binds to non polymorphic regi on of class I molecules;may in crease avidity of cell-cell in teracti onsAssociated with lymphoepithelioma-like carc in oma of lu ng (AJSP 2002;26:715)Positive stai ning (no

29、rmal):T cells (25-35% of mature peripheral T cells, most cytotoxicT cells, CD4/CD8+ thymocytes); NK cells (30%-which are also CD3 negative); corticalthymocytes (70-80%), epidermotrophic lymphocytes in mycosis fun goides (AJSP2002;26:450)Micro images:lymphoepithelioma-like care inoma of cervix-figure

30、 3, acutedemyeli nat ing diseaseMicro images (Mod Path subscribers):no dal cytotoxic T cell lymphomaRefere nee:Mod Path 2002;15:1131CD9May mediate platelet activati on and aggregati onAntibodies are used to purge bone marrow prior to peripheral stem cell bone marrowtran spla ntViral co-receptorPositive sta ining (n