1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEGramicidinCat. No.: HY-P0163CAS No.: 1405-97-6分式: CHNO分量: 1882.3作靶點: Bacterial作通路: Anti-infection儲存式: Powder -80C 2 years-20C 1 yearIn solvent -80C 6 months-20C 1 month溶解性數據體外實驗 DMSO : 100 mg/mL (53.13 mM)H2O : 90% corn oilSolub

2、ility: 2.5 mg/mL (1.33 mM); Clear solutionBIOLOGICAL ACTIVITY1/2 Master of Small Molecules 您邊的抑制劑師www.MedChemE物活性 Gramicidin種抗 肽，組裝成膜中的通道并增加其對陽離的滲透性。IC50 & Target Bacterial 1體外研究 Gramicidin is an antimicrobial peptide assembling as channels in membranes and increasing theirpermeability towards catio

3、ns. There is poor Gramicidin activity against E. coli and S. cerevisae as shownfrom the survival of microbes (%) over a range of Gramicidin concentrations, in contrast with the action ofGramicidin on S. aureus. The microbicidal activity occurs over a range of low Gramicidin and DODABconcentrations w

4、hich are not toxic to S. cerevisae 1.PROTOCOLCell Assay 1 Microbes and dispersions are mixed and interacted for one hour before being diluted up to 20,000-fold forplating of 0.1 mL of each in triplicate and incubation (24 hours for 37C) for CFU counting. Cell survival (%),taken as the meanstandard d

5、eviation, is plotted against DODAB and/or Gramicidin concentration. As acontrol for cell viability in the absence of DODAB or DODAB/Gramicidin dispersions, a standard bacterialsuspension is added to 0.264 M D-glucose solution, diluted, and spreaded on the agar plate 1.MCE has not independently confi

6、rmed the accuracy of these methods. They are for reference only.REFERENCES1. Ragioto DA, et al. Novel gramicidin formulations in cationic lipid as broad-spectrum microbicidal agents. Int J Nanomedicine. 2014 Jun30;9:3183-92.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use only.Tel: 400-82