Spawning,earlydevelopment,andfirstfeedinginthelemonpeel

angelfishCentropygeflavissimus(藍眼黃新娘)Aquaculture253(2006)270–2781.Introduction棘蝶魚科Pomacanthidae:7屬，70個種，分布廣泛。藍眼黃新娘2.Materialsandmethods2.1.AnimalsAllspeciesofCentropygestudiedtodatefromharemgroupsofonemaleandtwotofourfemales。lemonpeelangelfishCentropygeflavissimusweremaintainedinpairsin300Ltanksandfedtwicedaily(8amand4pm)withcommercialflakefoodandrawshrimporfish;Photoperiodandtemperatureweremanipulatedusinglighttimersandheatpumpstosimulateseasonalchanges.winterseason:10hlightand20C;summer:13hlightand28–30C;Springandfallconditions:11–12hlightand24–26C.Salinitywasmaintainedat32–36ppt,pH8.2,NO2andNH3小于0.03ppm.2.2.BehaviouralobservationsSpawningactivitywasrecordedondigitalvideoTape；Recordingsweremadeduringthefinal90minoflighteachevening.Aresearcherstartedtherecorderandimmediatelylefttheroomsoasnottodisturbthespawningpair。2.3.Embryocollection,development,andhatchingrate150目篩絹網集卵；5微米濾水，26度下于2L容器中孵化，PH8.2，鹽度32-36。2.4Larvalrearing150L玻璃鋼水槽培養。孵化6天后才微充氣。池中光線較弱，加綠藻至看不到池底止。從孵化6天后開始換水，每天早晨15%。每天加幾滴potassiumiodine（鉀碘）。2.5.Evaluationofliveprey對不同餌料培養效果進行評價。2.6.Experimentaldesign分組進行不同餌料下成活率實驗。6個培養容器，共1200粒卵用于溫度和營養對成活率影響的實驗研究。每天投喂兩次，每個容器約200尾。3.Results3.1.CourtshipandspawningA：產卵前；B：產卵；C：產卵后3.2.Fertilizationandhatchrate受精率大約90%。受精卵孵化率98%。26度下約15小時孵化。3.3.Embryodevelopment見下圖。A：受精后1小時B：受精后3小時，原腸期C：受精后8小時D：受精后10.5小時E：受精后14.5小時，孵化前。3.4.LarvaldevelopmentA：