教案2013~2014學年第1學期課程名稱臨床免疫學與檢驗開課學院XX檢驗學院開課教研室臨床免疫學檢驗授課教師XXX職稱技師授課班級2010級醫學檢驗學生人數102醫學院教務處制醫學院教案課程名稱ClinicalImmunology&ImmunologicalLaboratoryScience授課題目（章節或主題）ELISADetecthepatitisbvirus授課教師所屬二級學院KingmedCollegeofLaboratoryScience所屬教研室DepartmentofClinicalImmunology&ImmunologicalLaboratoryScience職稱Technician授課時間October11,2013,Thursday,Thesixthweek,section1,thefirstclass授課時數4classes授課班級Undergraduatelevel2010教學課型Theexperimentalclass教材名稱、作者、出版社及出版時間ChangqianZeng&ZhihuaTao.ExperimentGuideofClinicalImmunology&ImmunologicalLaboratoryScience.Chinesemedicinescienceandtechnologypress,2010,2.教學目標與要求：1、fullyUnderstandtheprinciple,operationprocessandattentionissuesoftheELISAmethodtodetectHepatitisBvirus.2、knowtheclinicalsignificanceofdetectionofHepatitisBvirus。主要知識點、重點與難點：Themainpoint：theprinciple,operationprocessofthedoubleantibody/antigensandwichmethod、neutralcompetitivemethod、competitiveinhibitionmethod.Thedifficultpoint:theoneisthecharacteristicsofneutralCompetitivemethodandcompetitiveinhibitionmethod,theotheroneistheclinicalsignificanceofdifferentresults.教學方法（請打√選擇）：講授法□√討論法□√演示法□√自學輔導法□練習法(習題或操作)√讀書指導法□□案例法□□教學媒體（請打√選擇）：教材□板書□√實物□√標本□√掛圖□模型□投影□幻燈□錄像□CAI（計算機輔助教學）□√教學過程設計（包括講授內容、講授方法、時間分配、媒體選用、板書設計等）：講授內容講授方法、時間分配、手段一、FivedifferentantibodiesandantigensoftheHepatitisBandthedetectionmethodHBsAg:DoubleantibodysandwichmethodHBsAb:DoubleantigensandwichmethodHBeAg:DoubleantibodysandwichmethodHBeAb:NeutralCompetitivemethodHBcAb:Competitiveinhibitionmethod2minutesIntroducingthemaincontent二、introducethedetectionkit:3kindsofessentialreagents:①Thecoatingantigenorantibody----immuneadsorbent②Theenzymelabeledantigenorantibody----enzymeconjugates③Thesubstrateoftheenzymereaction----thesubstrate5minutes三、Theprincipleofthedoubleantibody/antigensandwichmethod、neutralcompetitivemethod、competitiveinhibitionmethod.Theprincipleofdoubleantibodysandwichmethod(two-stepsmethod)Theprincipleofdoubleantibodysandwichmethod(one-stepsmethod)Thedifferencesbetweentwo/onestepsmethodTheprincipleofdoubleantigensandwichmethodTheprincipleofcompetitiveinhibitionmethodTheprincipleofneutralcompetitiveinhibitionmethodThepictures

andanimationwillmake

students

moreimpressive.5minutes2minutes4minutes2minutes3minutes2minutes四、DetectionStepsandattentionnotes:10minutes五、resultandclinicalsignificanceHBsAganti-HBsHBeAganti-HBeanti-HBcClinicsignificance+----LatencyperiodofacutehepatitisBvirusinfection+-+--earlyperiodofacutehepatitisBvirusinfectionanditcancontagioustootherpeople.+-+-+AcuteorchronichepatitisBinfection,viralreplicationisactive.Infectivityisstrong.（大三陽）+---+AcuteorchronichepatitisBinfection+-+++AcuteorchronichepatitisBinfection.HBeAgconversetoanti–HBewhichhascertaininfectivity+--++AcuteorchronichepatitisBinfection.Virusreplicationreducesandinfectiousisweak.（小三陽）++-++Intherecoveryperiod,bodyproducesimmunity.---++Intherecoveryperiodorhasapreviousinfectionhistory.-+-++Intherecoveryperiodandbodyhasimmunity.-+---BodyhasbeenvaccinatedbyhepatitisBvaccine,orintherecoveryperiod,orhasimmunity.----+ThewindowperiodofacutehepatitisBvirusinfection,orhasapreviousinfectionhistory.-+--+Intherecoveryperiod,bodyhasimmunity.-----Noinfection,novaccinationornoimmunity5minutes提問、課堂討論等師生互動的設計：1、Whatistheprincipleofdoubleantibody(orantigen)methodandcompetitivemethod?2、Whatarethedifferencesbetweentwo-stepsmethodandone-stepmethod?教學小結、復習思考及作業題布置：summarize：FivedifferentantibodiesandantigensoftheHepatitisBandthedetectionmethod.Introducingthedetectionkit:3kindsofessentialreagents.Theprincipleofthedoubleantibody/antigensandwichmethod、neutralcompetitivemethod、competitiveinhibitionmethod.DetectionStepsandattentionnotes.ResultandclinicalsignificancesQuestures：1、Whatistheprincipleofdoubleantibody(orantigen)methodandcompetitivemethod?2、Whatarethedifferencesbetweentwo-stepsmethodandone-stepmethod?教學中的創新點（加強基礎與臨床聯系、外語運用、啟發學生思維、指導學生自學、介紹學科新進展等方面）：1、Focusontheconnectionoftheoryandpractice.Questionsandinteractiveteachingwillinspirestudentstothink.2,Usingthegraphicsandanimationtodescribetheexperimentalprinciplewhichwillhelpthestudentstounderstandtheteachingcontent.3、Summarizetheprincipleoftheexperimentsandthecommonpoint,thedifferencesoftwostepmethodandonestepmethod.Simplifiedtheexperimentalstepsandhelpstudentsunderstandandmemorytheknowledge.參考資料（包括輔助教材、參考書、文獻等）：1.《ClinicalImmunologyExamination》（Thesecondedition），ChinamedicinescienceandTechnologyPress；2.《MedicalImmunity》（TenthEdition），ScienceandTechnologyPress；教研室意見教研室主任簽章：年月日教學后記（即教學實施情況總結分析，在課程結束后填寫）