Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemEAutophagyinducer7Cat.No.:HY-171047CASNo.:944159-20-0分?式:C??H??FN?OS分?量:410.55作?靶點:Apoptosis;Autophagy;DNA/RNASynthesis;Akt;mTOR作?通路:Apoptosis;Autophagy;CellCycle/DNADamage;PI3K/Akt/mTOR儲存?式:4°C,sealedstorage,awayfrommoistureandlight*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoistureandlight)溶解性數據體外實驗DMSO:50mg/mL(121.79mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM2.4358mL12.1788mL24.3576mL5mM0.4872mL2.4358mL4.8715mL10mM0.2436mL1.2179mL2.4358mL請根據產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復凍融造成的產品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoistureandlight)。-80°C儲存時，請在6個?內使?，-20°C儲存時，請在1個?內使?。BIOLOGICALACTIVITY?物活性Autophagyinducer7(CompoundSSA)?種?噬(Autophagy)和凋亡(Apoptosis)誘導劑。Autophagyinducer7通過抑制Akt/mTOR信號傳導和下游蛋?的表達來激活?噬。Autophagyinducer7抑制DNA合成(DNAsynthesis)并導致G0-G1細胞周期停滯。Autophagyinducer7抑制腫瘤細胞?長。體外研究Autophagyinducer7(72h)inhibitsthegrowthofhumanlungadenocarcinomacelllines,withIC50sof1.95μM(HOP-62),4.72μM(A549),3.05μM(H1299)[1].1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEAutophagyinducer7(0-10μM,24h)inducesapoptosisinHOP-62,A549,H1299cells,butnotsignificantasSulindacsulfide(HY-B1786)andStaurosporine(HY-15141)[1].Autophagyinducer7(0-7.5μM,24h)inhibitsDNAsynthesisinHOP-62,A549,H1299cells,andincreasesthepercentageofcellsintheG0-G1phaseofthecellcycle[1].Autophagyinducer7(0-7.5μM,24h)inducesautophagyandincreasesautophagicfluxbyinhibitingAkt/mTOR/p70S6ksignalinginA549cells[1].ApoptosisAnalysis[1]CellLine:HOP-62,A549,H1299cellsConcentration:2.5,5,7.5,10μMIncubationTime:24hResult:Inducedcaspase3/7activationdose-dependently.InducedcleavedPARPexpressionat10μM.IncreasedAnnexin-VsurfacestainingandPIlabeling.CellAutophagyAssay[1]CellLine:HOP-62,A549,H1299cellsConcentration:2.5,5,7.5,10μMIncubationTime:24hResult:Induceddose-andtime-dependentcleavageofcytosolicLC3-Itoautophagosome-associatedLC3-II.Decreasedp62expression.Displayedastrikingaccumulationofvesicle-likestructureswithinthecytoplasm.Increasedboththenumberandsizeofacidicvesicleswithinthecytoplasm.REFERENCES[1].1.GurpinarE,etal.AnovelsulindacderivativeinhibitslungadenocarcinomacellgrowththroughsuppressionofAkt/mTORsignalingandinductionofautophagy.MolCancerTher.2013May;12(5):663-74.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplicati