1、熒光素名稱激發(fā)波長(zhǎng) (nm)發(fā)射波長(zhǎng) (nm)備注1,5 IAEDANS336490 1,8-ANS372480 4-Methylumbelliferone385502 5-carboxy-2,7-dichlorofluorescein504529 5-Carboxyfluorescein (5-FAM)492518 5-Carboxynapthofluorescein (pH 10)512/598563/668Ratio Dye, pH5-Carboxytetramethylrhodamine (5-TAMRA)542568 5-F

2、AM (5-Carboxyfluorescein)492518 5-HAT (Hydroxy Tryptamine)370-415520-540 5-Hydroxy Tryptamine (HAT)370-415520-540 5-ROX (carboxy-X-rhodamine)578567 604591 5-TAMRA (5-Carboxytetramethylrhodamine)548542 552568 6-Carboxyrhodamine 6G518543 6-CR 6G518543 6-JOE520548

3、0;7-Amino-4-methylcoumarin351430 7-Aminoactinomycin D (7-AAD)546647 7-Hydroxy-4-methylcoumarin360449, 455 9-Amino-6-chloro-2-methoxyacridine412, 430471, 474 ABQ344445 Acid Fuchsin540630 ACMA (9-Amino-6-chloro-2-methoxyacridine)412, 430471, 474 Acridine Orange + DNA

4、502526 Acridine Orange + RNA460650 Acridine Orange, both DNA & RNA440-480520-650 Acridine Red455-600560-680 Acridine Yellow470550 Acriflavin436520 Acriflavin Feulgen SITSA355-425460 Aequorin (Photoprotein) 466PhotoproteinAFPs - AutoFluorescent Protein - (Q

5、uantum Biotechnologies) see sgGFP, sgBFP   Alexa Fluor 350346342 442441 Alexa Fluor 430431540 Alexa Fluor 488495, 492519, 520 Alexa Fluor 532531, 532553, 554 Alexa Fluor 546556, 557572, 573 Alexa Fluor 568577, 578603 Alexa Fluor 594590, 594617, 618 A

6、lexa Fluor 633632650 Alexa Fluor 647647666 Alexa Fluor 660668698 Alexa Fluor 680679702 Alizarin Complexon530-560, 580580, 624-645 Alizarin Red530-560580 Allophycocyanin (APC)630, 645655, 660 AMC, AMCA-S345445 AMCA (Aminomethylcoumarin)345347 425444 AMCA-X

7、353442 Aminoactinomycin D555655 Aminocoumarin346350 442445 Aminomethylcoumarin (AMCA)345347 425444 Anilin Blue 600 Anthrocyl stearate360-381446 APC (Allophycocyanin)630, 645655, 660 APC-Cy7625-650755 APTRA-BTC = Ratio Dye, Zn2+466/380520/530Ratio Dye, Zn2

8、+APTS424505 Astrazon Brilliant Red 4G500585 Astrazon Orange R470540 Astrazon Red 6B520595 Astrazon Yellow 7 GLL450480 Atabrine436490 ATTO-TAG CBQCA465560 ATTO-TAG FQ486591 Auramine460550 Aurophosphine G450580 Aurophosphine450-490515 BAO 9 (Bisam

9、inophenyloxadiazole)365395 BCECF (high pH)492, 503520,528 BCECF (low pH)482520 Berberine Sulphate430550 Beta Lactamase409447, 520 BFP blue shifted GFP (Y66H)Blue Fluorescent Protein381, 382, 383445, 447, 448blue shifted GFP (Y66H)Blue Fluorescent ProteinBFP/GFP FRET 

10、60; Bimane398490 Bisbenzamide360461 Bisbenzimide (Hoechst)360461 bis-BTC = Ratio Dye, Zn2+455/405529/505Ratio Dye, Zn2+Blancophor FFG390470 Blancophor SV370435 BOBO -1462481 BOBO -3570602 Bodipy 492/515490515 Bodipy 493/503533549 Bodipy 500/510509515

11、 Bodipy 505/515502510 Bodipy 530/550528547 Bodipy 542/563543563 Bodipy 558/568558569 Bodipy 564/570564570 Bodipy 576/589579590 Bodipy 581/591584592 Bodipy 630/650-X625642 Bodipy 650/665-X647665 Bodipy 665/676605676 Bodipy Fl504, 505511, 513 

12、;Bodipy FL ATP505514 Bodipy Fl-Ceramide505511 Bodipy R6G SE528547 Bodipy TMR542574 Bodipy TMR-X conjugate544573 Bodipy TMR-X, SE544570 Bodipy TR589617 Bodipy TR ATP591620 Bodipy TR-X SE588616 BO-PRO -1462481 BO-PRO -3544570 Brilliant Sulphoflavi

13、n FF430520 BTC - Ratio Dye Ca2+464/401533/529Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+459/417517/532Ratio Dye, Zn2+Calcein494517 Calcein Blue373440 Calcium Crimson588, 589611, 615 Calcium Green501, 506531 Calcium Green-1 Ca2+ Dye506531Ca2+ DyeCalcium Green-2 Ca2+506/503536Ca2+Ca

14、lcium Green-5N Ca2+506532Ca2+Calcium Green-C18 Ca2+509530Ca2+Calcium Orange549575576 Calcofluor White385, 395, 405437, 440, 445 Carboxy-X-rhodamine (5-ROX)576601 Cascade Blue377398399 420423 Cascade Yellow399400 550552 Catecholamine410470 CCF2 (GeneBlazer)  &#

15、160;CFDA494520 CFP - Cyan Fluorescent Protein430, 433, 436, (453)474, 475, 476, (501)Cyan Fluorescent ProteinCFP/YFP FRET   Chlorophyll480650 Chromomycin A436-460470 Chromomycin A445575 CL-NERF (Ratio Dye, pH)504/514540Ratio Dye, pHCMFDA494520 Coelenterazine C

16、a2+ Dye, bioluminescence(429)465Ca2+ Dye, bioluminescence, native moleculeCoelenterazine cp (Ca2+ Dye,)(430)442Ca2+ Dye, bioluminescenceCoelenterazine f(437)473Ca2+ Dye, bioluminescenceCoelenterazine fcp 452Ca2+ Dye, bioluminescenceCoelenterazine h(437)464Ca2+ Dye, bioluminescenceCoelenterazine

17、 hcp(433)444Ca2+ Dye, bioluminescenceCoelenterazine ip 441Ca2+ Dye, bioluminescenceCoelenterazine n(431)467Ca2+ Dye, bioluminescenceCoelenterazine O460575 Coumarin Phalloidin387470 C-phycocyanine   CPM Methylcoumarin384469MethylcoumarinCTC400-450602 CTC Formazan

18、60;  Cy2489506 Cy3.1 8554568 Cy3.5581598 Cy3514552554 566570 Cy5.1 8649666 Cy5.5675695 Cy5649666670 Cy7710, 743767, 805 Cyan GFP433 (453)475 (501) cyclic AMP Fluorosensor (FiCRhR)500517 CyQuant Cell Proliferation Assay480520Cell Proliferati

19、on AssayDabcyl453  Dansyl340578 Dansyl Amine337517 Dansyl Cadaverine335518 Dansyl Chloride372518 Dansyl DHPE336517 Dansyl fluoride356none DAPI359461 Dapoxyl403580 Dapoxyl 2374574 Dapoxyl 3373574 DCFDA504529 DCFH (Dichlorodihydrofluores

20、cein Diacetate)505535 DDAO463607 DHR (Dihydorhodamine 123)505534 Di-4-ANEPPS496705 Di-8-ANEPPS (non-ratio)488498 605713 DiA (4-Di-16-ASP)456591 Dichlorodihydrofluorescein Diacetate (DCFH)505535 DiD - Lipophilic Tracer644665Lipophilic TracerDiD (DiIC18(5)644665 

21、;DIDS341415 Dihydorhodamine 123 (DHR)505535 DiI (DiIC18(3)549, 551565 Dinitrophenol349  DiO (DiOC18(3)484, 487501, 502 DiR748780Lipophilic TracerDiR (DiIC18(7)750779 DM-NERF (high pH)497/510540Ratio Dye, pHDNP349  Dopamine340490-520 DsRed558583Red fl

22、uorescent proteinDTAF494520 DY-630-NHS621660Hemicyanelabel for proteins and DNADY-635-NHS634664Hemicyanelabel for proteins and DNAEBFP383447Enhanced Blue Fluorescent ProteinECFP436474Enhanced Cyan Fluorescent ProteinEGFP488, 498507, 516Enhanced Green Fluorescent ProteinELF 97345530 Eosin52

23、4545 Erythrosin529, 532554, 555 Erythrosin ITC529555 Ethidium Bromide510, 523595, 605 Ethidium homodimer -1 (EthD-1)528617 Euchrysin430540 EukoLight   Europium (III) chloride   EYFP513, 520527, 532Enhanced Yellow Fluorescent ProteinFast Blu

24、e360440 FDA494520 Feulgen (Pararosaniline)570625 FIF (Formaldehyd Induced Fluorescence)405433 FITC490, 494520, 525 FITC Antibody493517 Flazo Orange375-530612 Fluo-3480 506, 506520, 527 Fluo-4494516 Fluorescein (FITC)490, 494520, 525 Fluorescein Diace

25、tate494520 Fluoro-Emerald495524 Fluoro-Gold (Hydroxystilbamidine)361536 Fluor-Ruby555582 FluorX494520 FM 1-43479598 FM 4-46515640 Fura Red (high pH)572657 Fura Red/Fluo-3   Fura-2, high calcium335505Excitation ratio dyeFura-2, low calcium363512E

26、xcitation ratio dyeFura-2/BCECF   Genacryl Brilliant Red B520590 Genacryl Brilliant Yellow 10GF430485 Genacryl Pink 3G470583 Genacryl Yellow 5GF430475 GeneBlazer (CCF2)   GFP (S65T)498516 GFP red shifted (rsGFP)498516 GFP wild type, non-UV

27、excitation (wtGFP)475509 GFP wild type, UV excitation (wtGFP)395509 GFPuv385508 Gloxalic Acid405460 Granular Blue355425 Haematoporphyrin530-560580 Hoechst 33258345487 Hoechst 33342347483 Hoechst 34580392440 HPTS355465 Hydroxycoumarin325-360386-455

28、60;Hydroxystilbamidine (FluoroGold)361536 Hydroxytryptamine400530 Indo-1, high calcium330401Emission ratio dyeIndo-1, low calcium346475Emission ratio dyeIndodicarbocyanine (DiD)644665 Indotricarbocyanine (DiR)748780 Intrawhite Cf360430 JC-1514529 JO-JO-1530545 JO-P

29、RO-1532544 LaserPro795812 Laurodan355460 LDS 751 (DNA)543712 LDS 751 (RNA)590607 Leucophor PAF370430 Leucophor SF380465 Leucophor WS395465 Lissamine Rhodamine572, 577591, 592 Lissamine Rhodamine B577592 LIVE/DEAD Kit Animal CellsCalcein/Ethidium homo

30、dimer494528517617for more details referto LOLO-1566580 LO-PRO-1568581 Lucifer Yellow425, 428528, 536, 540 Lyso Tracker Blue373422 Lyso Tracker Blue-White466536 Lyso Tracker Green504, 534511, 551 Lyso Tracker Red490516 Lyso Tracker Yellow551576 LysoSensor Blue3

31、74424 LysoSensor Green442505 LysoSensor Yellow/Blue384540 Mag Green507531 Magdala Red (Phloxin B)524600 Mag-Fura Red483/427659/631Ratio Dye, Mg2+Mag-Fura-2369/329369/330508511/491Ratio Dye Ca2+Ratio Dye Mg2+Mag-Fura-5369/330369/332505/500505/482Ratio Dye, Ca2+Ratio Dye, Mg2+

32、Mag-Indo-1349/328349/330480/390480/417Ratio Dye, Ca2+Ratio Dye, Mg2+Magnesium Green506, 507531 Magnesium Orange550575 Malachite Green628  Marina Blue362459 Maxilon Brilliant Flavin 10 GFF450495 Maxilon Brilliant Flavin 8 GFF460495 Merocyanin555578 Methoxycouma

33、rin360410 Mitotracker Green FM490516 Mitotracker Orange551576 Mitotracker Red578599 Mitramycin450470 Monobromobimane398490 Monobromobimane (mBBr-GSH)398500 Monochlorobimane380461 MPS (Methyl Green Pyronine Stilbene)364395 NBD466539 NBD Amine450530

34、60;Nile Red515-555, 559590, 640 Nitrobenzoxadidole465510-650 Noradrenaline340490-520 Nuclear Fast Red289-530580 Nuclear Yellow365495 Nylosan Brilliant Iavin E8G460510 Oregon Green503522 Oregon Green 488-X494517 Oregon Green503522 Oregon Green 488490, 4935

35、14, 520 Oregon Green 500497517 Oregon Green 514506526 Pacific Blue405455 Pararosaniline (Feulgen)570625 PBFI340/380420Excitation ratio dyePE-Cy5488670 PE-Cy7488755, 767 PerCP488675 PerCP-Cy5.5488710 PE-TexasRed Red 613488613 Phloxin B (Magdala Red)52

36、4600 Phorwite AR360430 Phorwite BKL370430 Phorwite Rev380430 Phorwite RPA375430 Phosphine 3R465565 PhotoResist365610 Phycoerythrin B PE546-565575 Phycoerythrin R PE565578 PKH26 (Sigma)551567 PKH67496520ChromaPMIA341376 Pontochrome Blue Black535-

37、553605 POPO-1433457 POPO-3533574 PO-PRO-1435455 PO-PRO-3539567 Primuline410550 Procion Yellow470600 Propidium Iodid (PI)(305), 536, 538617 PyMPO412, 415561, 564, 570 Pyrene360387 Pyronine410540 Pyronine B540-590560-650 Pyrozal Brilliant Fla

38、vin 7GF365495 QSY 7560  Quinacrine Mustard440510 Red 613 PE-TexasRed488613 Resorufin571584, 585 RH 414532716 Rhod-2552576 Rhodamine550573 Rhodamine 110496, 497520 Rhodamine 123507529 Rhodamine 5 GLD470565 Rhodamine 6G525555 Rhodamine B

39、540625 Rhodamine B 200523-557595 Rhodamine B extra550605 Rhodamine BB540580 Rhodamine BG540572 Rhodamine Green502527 Rhodamine Phallicidine558, 542575, 565 Rhodamine Phalloidine542565 Rhodamine Red570590 Rhodamine WT530555 Rose Bengal525, 540550-600&

40、#160;R-phycocyanine   R-phycoerythrin (PE)565578 rsGFP473509red shifted GFP (S65T)S65A471504 S65C479507 S65L484510 S65T488511 Sapphire GFP395511 SBFI340/380420Excitation ratio dyeSerotonin365520-540 Sevron Brilliant Red 2B520595 Sevron Brilliant

41、 Red 4G500583 Sevron Brilliant Red B530590 Sevron Orange440530 Sevron Yellow L430490 sgBFP387450 sgBFP (super glow BFP)387450Quantums SuperGloGFPAFPssgGFP474488 sgGFP (super glow GFP)474509Quantums SuperGloGFPAFPsSITS336436Ion ChannelsSITS (Primuline)395-425450 SIT

42、S (Stilbene Isothiosulphonic Acid)365460 SNAFL calcein506/535535/620Ratio Dye, pHSNAFL-1508/540543/623Ratio Dye, pHSNAFL-2514/543546/630Ratio Dye, pHSNARF calcein552/574590/629Ratio Dye, pHSNARF1576/548635/587Excitation and emission ratio dyeSodium Green506, 507532Na+, K+SpectrumAqua433,/53480/

43、55VysisSpectrumGreen497/30, 509/31538/44, 524/56VysisSpectrumOrange559/38, 560588/48VysisSpectrum Red587, 587/35612, 612/51 SPQ (6-methoxy-N-(3-sulfopropyl)quinolinium)344443 Stilbene335440 Sulphorhodamine B can C520595 Sulphorhodamine G Extra470570 SYTO 11508, 510527, 530Dy

44、e for DNA, RNASYTO 12499, 500522, 519Dye for DNA, RNASYTO 13488, 491509, 514Dye for DNA, RNASYTO 14517, 521549, 547Dye for DNA, RNASYTO 15516, 518546, 555Dye for DNA, RNASYTO 16488, 494518, 525Dye for DNA, RNASYTO 17621634Dye for DNASYTO 18490, 493507, 527Dye for DNA, RNASYTO 20512530Dye for DNASYTO

45、 21494517Dye for DNASYTO 22515535Dye for DNASYTO 23499520Dye for DNASYTO 24490515Dye for DNASYTO 25521556Dye for DNASYTO 40420441Dye for DNASYTO 41430454Dye for DNASYTO 42433460Dye for DNASYTO 43436467Dye for DNASYTO 44446471Dye for DNASYTO 45452484Dye for DNASYTO 59622645Dye for DNASYTO 60652678Dye

46、 for DNASYTO 61628645Dye for DNASYTO 62652676Dye for DNASYTO 63657673Dye for DNASYTO 64599619Dye for DNASYTO 80531545Nucleic Acid StainSYTO 81530544Nucleic Acid StainSYTO 82541560Nucleic Acid StainSYTO 83543559Nucleic Acid StainSYTO 84567582Nucleic Acid StainSYTO 85567583Nucleic Acid StainSYTOX Blue

47、445470Nucleic Acid StainSYTOX Green504523Nucleic Acid StainSYTOX Orange547570Nucleic Acid StainTetracycline390-425525-560 Tetramethylrhodamine (TRITC)555576 Texas Red595620 Texas Red-X conjugate595615 Thiadicarbocyanine (DiSC3)651, 653674, 675 Thiazine Red R596615 Thiaz

48、ole Orange510530 Thioflavin 5430550 Thioflavin S430550 Thioflavin TCN350460 Thiolyte370-385477-488 Thiozole Orange453480 Tinopol CBS (Calcofluor White)390430 TMR550573 TO-PRO-1515531 TO-PRO-3644657 TO-PRO-5747770 TOTO-1514531, 533 TOTO-3642

49、660 TriColor (PE-Cy5)(488) 650667 TRITC TetramethylRodamineIsoThioCyanate550573 True Blue365425 TruRed490695 Ultralite656678 Uranine B420520 Uvitex SFC365435 wt GFP395 (475)508wild type GFPWW 781605639 X-Rhodamine580605 XRITC582601 Xylene Orange

50、546580 Y66F360508 Y66H360442 Y66W436485 Yellow GFP513527Yellow shifted Green Fluorescent ProteinYFP513, 520527, 532Yellow Fluorescent ProteinYO-PRO-1491506 YO-PRO-3613629 YOYO-1491508, 509 YOYO-3612631 常用熒光染料的激發(fā)及發(fā)射波長(zhǎng)Fluorescent Dye（熒光染料）Excitation（激發(fā)波長(zhǎng)nm&

51、#160;）Emission 發(fā)射波長(zhǎng)nm ） Cy2 TM                       489         506 GFP(Red Shifted)    &

52、#160;    488         507 YO-PRO TM -1         491         509 YOYO TM -1       &

53、#160; 491         509 Calcein                        494         517 FITC&#

54、160;                       494         518 FluorX TM         494   

55、60;     519 Alexa TM 488         490         520 Rhodamine 110         496       

56、;  520 ABI,5-FAM         494         522 Oregon Green TM 500        503         522 O

57、regon Green TM 488          496         524 RlboGreen TM         500         525 Rhod

58、amine Green TM         502         527 Rhodamine123         507         529 Magnesium Green

59、0;TM         506         531 Calcium Green TM         506         533 TO-PRO TM -1 &#

60、160;       514         533 TOTO?-1         514         533 ABI,JOE         

61、             520         548 BODIPY?530/550         530         550 Dil   &

62、#160;                    549         565 BODIPY?TMR         542       

63、  568 BODIPY?558/568         558         568 BODIPY?564/570         564         570 Cy3 TM

64、                         550         570 Alexa TM 546         555 

65、;        570 TRITC                          547         572 Magnesium 

66、Orange TM 550         575 Phycoerythrin,R B 565         575 Rhodamine Phalloidin 550         575 Calcium Orange

67、0;TM 549         576 Pyronin Y         555         580 Rhodamine B         555         580 ABI,TAMRA         560         582 Rhodamine Red TM 570         590 Cy3.