1、Copyright 2009HAPSITE ER/Smart Plus Training HAPSITE ER/Smart Plus 培培 訓訓Headspace Sampling System 頂空采樣系統Copyright 20092HSS Module Outline HSS 模件概要模件概要 HSS Assembly HSS 裝配裝配 HSS Theory HSS 原理原理 HSS Internal Standards HSS 內部標準內部標準 Preparing Blanks/Samples 準備空樣準備空樣/樣品樣品 Running Analyze 運行分析運行分析 Quantit

2、ation Reports 定量報告定量報告 Contamination Issues 污染問題污染問題Copyright 20093What is the Headspace Sampling System 什么是頂空采樣系統什么是頂空采樣系統 HAPSITE accessory HAPSITE 輔件輔件 Sampling system for: 采樣系統用于采樣系統用于: Water 水水 Soil 土壤土壤 Liquids 液體液體 Detects VOCs 檢測檢測 VOCs Qualitative methods 定性方法定性方法 Quantitative methods定量方法定量

3、方法 PPB to PPT range PPB 至至 PPT 量程量程Copyright 20094HSS Assembly HSS 裝配裝配 Insert a charged battery into the HAPSITE 將充足電的電池插入將充足電的電池插入 HAPSITE Disconnect the HAPSITE power supply cord 斷開斷開 HAPSITE 的電源電纜的電源電纜連接連接Copyright 20095Connecting the Y-Cable for Stationary Use 為臺式用法連接為臺式用法連接 Y - 電纜電纜 As the nam

4、e implies, the cable forms a Y 正如其名正如其名, 電纜呈電纜呈 Y 形形Joined end 連接端連接端Split ends 分叉端分叉端Copyright 20096Connecting the Y-Cable for Stationary Use為臺式用法連接為臺式用法連接 Y - 電纜電纜 Connect the joined end to the HAPSITE power supply cord 將連接端連接至將連接端連接至 HAPSITE 的電源電纜的電源電纜NOTE: Align the dots on the cable with the do

5、ts on the cord 注注: 對準電纜上的點與電源線上的點對準電纜上的點與電源線上的點Copyright 20097Connecting the Y-Cable for Stationary Use為臺式用法連接為臺式用法連接 Y - 電纜電纜 Connect one split end of the cord to the HAPSITE 連接電纜的一個分叉端至連接電纜的一個分叉端至 HAPSITE Connect the other split end to the Headspace Sampling System 連接另一個分叉端至頂空采樣系統連接另一個分叉端至頂空采樣系統NO

6、TE: Align the dots on the cable with the dots on the instruments to properly connect 注注: 將電纜上的點與儀器上的點對準將電纜上的點與儀器上的點對準, 直至妥善地連接上直至妥善地連接上Copyright 20098Transfer Line 傳輸線傳輸線 Provides communication between the HAPSITE ER/Smart Plus and the HSS 提供提供 HAPSITE ER/Smart Plus 與與 HSS 之間的通訊之間的通訊 Sample travels

7、through the transfer line to the HAPSITE ER/Smart Plus 樣品經過傳輸線傳送給樣品經過傳輸線傳送給HAPSITE ER/Smart Plus HAPSITE ER Transfer Line 傳輸線傳輸線HAPSITE Smart Plus Transfer Line 傳輸線傳輸線Copyright 20099Transfer Line 傳輸線傳輸線 Encompassed by a foam sleeve 外包泡沫套管外包泡沫套管 Provides insulation 提供絕緣提供絕緣 Reduces power requirements

8、降低功率要求降低功率要求 Directional 方向性方向性 Multi-connector end connects to the HAPSITE ER Universal Interface 多接頭端連接至多接頭端連接至 HAPSITE ER 的通用接口的通用接口 For the HAPSITE Smart Plus, the direction of the transfer line is labeled directly on it HAPSITE Smart Plus 的傳輸線的傳輸線方向直接標注在它的上面方向直接標注在它的上面Copyright 200910Inserting

9、the Transfer Line 插入傳輸線插入傳輸線 For the HAPSITE ER, plug the end with the single LEMO connection into the HSS 對于對于 HAPSITE ER, 將帶有單將帶有單個個LEMO 接頭插入接頭插入 HSS Align the red dots 對準紅點對準紅點 For the HAPSITE Smart Plus, plug the side labeled This End to Accessory into the HSS 對于對于 HAPSITE Smart Plus, 將邊上標志將邊上標志

10、此端至附件端此端至附件端的的一端插入一端插入 HSS Align the red dots 對準紅點對準紅點Copyright 200911Inserting the Transfer Line 插入傳輸線插入傳輸線 Remove the probe from the HAPSITE ER/Smart Plus 從從 HAPSITE ER/Smart Plus 上卸下上卸下探頭探頭 To attach the transfer line to the HAPSITE ER 連接傳輸線至連接傳輸線至 HAPSITE ER Screw the Universal Bulkhead connecti

11、on into place 擰上擰上通用的防水通用的防水連接件連接件 Align the red dots 對準紅點對準紅點 Insert the LEMO connection 插插上上LEMO 接頭接頭 To attach the transfer line to the HAPSITE Smart Plus 連接傳輸線至連接傳輸線至 HAPSITE Smart Plus Align the red dots 對準紅點對準紅點 Insert the LEMO connection 插插上上 LEMO 接頭接頭 HAPSITE ER View HAPSITE ER 視圖 Copyright

12、200912Portable HSS Use 便攜式便攜式 HSS 的用法的用法 The HSS is designed for portable use HSS 設計于便攜式應用設計于便攜式應用Requires a battery 需用電池需用電池 Open the front door of the HSS 打開打開 HSS 的前門的前門 Insert the battery into the slot 將電池插入槽內將電池插入槽內 The arrow on the battery will be facing the battery release button電池上的箭頭指向電池釋放按

13、鈕電池上的箭頭指向電池釋放按鈕 The INFICON label will be in the lower left-hand corner INFICON 的標簽位于左下角的標簽位于左下角Copyright 200913Portable HSS Use 便攜式便攜式 HSS 的用法的用法 Push battery into slot until a “click” is heard 將電池推入槽中將電池推入槽中, 直到聽見直到聽見 “咔噠聲咔噠聲” Verify that the battery is securely fastened 證實電池已被牢固地固定住證實電池已被牢固地固定住Co

14、pyright 200914Nitrogen Canister 氮氣瓶氮氣瓶 Insert the nitrogen canister插入氮氣瓶插入氮氣瓶Copyright 200915Turning on the HSS 將將 HSS 的電源的電源 on Press down on the POWER switch 按下按下電源電源 開關開關 The POWER switch is a toggle switch 電源開關電源開關是扳鈕開關是扳鈕開關 Once pressed, it will return to its original position 一旦按下一旦按下, 它將回到原位它

15、將回到原位 The word POWER will illuminate 文字指示燈文字指示燈 POWER 亮亮 If the Headspace is powered by the HAPSITE power supply, the RMT POWER (Remote Power) will also illuminate 如頂空使用如頂空使用 HAPSITE 的電源的電源, RMT POWER (遙控電源遙控電源) 也亮也亮 Close the front door 關上前門關上前門Copyright 200916Preparing Analyze 準備分析準備分析 The HAPSITE

16、 ER/Smart Plus will automatically begin preparing a Headspace method HAPSITE ER/Smart Plus 將自動將自動開始準備頂空方法開始準備頂空方法 All components will be heated to their set point temperature 全部組件將加熱至它們的設定溫度全部組件將加熱至它們的設定溫度 AutoTune will be run 自動調諧將運行自動調諧將運行 Concentrator Cleanout will be run if a concentrator is ins

17、talled 如安裝濃縮器將運行濃縮器清理如安裝濃縮器將運行濃縮器清理Copyright 200917HSS Theory HSS 原理原理 VOCs collect as a gas in the headspace above the liquid or solid sample 在液體或固體樣品上在液體或固體樣品上, VOCs作作為氣體收集在頂空中為氣體收集在頂空中 Samples need to heat for 15-20 minutes to reach equilibrium 需將樣品加熱需將樣品加熱15-20 分鐘分鐘, 達到達到平衡平衡 Equilibrium occurs

18、when the rate of VOCs leaving the sample is equal to the rate of VOCs returning to the sample 平衡發生于當平衡發生于當 VOCs 離開樣品離開樣品與返回樣品的速率相等時與返回樣品的速率相等時 Vapor Phase蒸氣相蒸氣相Liquid or Solid Phase 液相或固體液相或固體Copyright 200918Determining Equilibration Times 確定平衡時間確定平衡時間AREA 面積面積Copyright 200919HSS Sampling HSS 采樣采樣 T

19、he Headspace needle is concentric and has two openings 頂空針頭是同心的頂空針頭是同心的, 有兩個開口有兩個開口 Sample is drawn in from the top opening 樣品樣品從頂開口引入從頂開口引入 Nitrogen is bubbled out of the bottom opening 氮氮從底開口涌出從底開口涌出Copyright 200920Sensitivity Tree 靈敏度樹狀圖靈敏度樹狀圖High Concentration高濃度高濃度LowConcentration低濃度低濃度LowSensi

20、tivity低靈敏度低靈敏度HighSensitivity高靈敏度高靈敏度ER_HSS_PPT_StandardHSS_Tri-Bed_PPT_Standard (low 低低 ppt)ER_HSS_PPB_StandardHSS_Loop_PPB_Standard(low 低低 ppb high高高 ppt)ER_Air_Tri-Bed_PPB_StandardAir_Tri-Bed_PPB_Standard(low 低低 ppb high 高高 ppt)Survey 速查速查HAPSITE ER: 1 ppmHAPSITE Smart Plus: 10 ppmER_Air_Tri-Bed_

21、PPM_StandardAir_Tri-Bed_PPM_Standard(low 低低 ppm high 高高 ppb)Copyright 200921HSS Internal Standards HSS 內部標準內部標準 The matrix of the internal standard must match the sample matrix 內部標準的基質必須與樣品的基質匹配內部標準的基質必須與樣品的基質匹配 Liquid samples use a liquid internal standard 液體樣品使用液體的內部標準液體樣品使用液體的內部標準 Gas samples use

22、 a gas internal standard 氣體樣品使用氣體的內部標準氣體樣品使用氣體的內部標準 TRIS and BPFB are gas standards TRIS 和和 BPFB 是氣體標準是氣體標準 These standards will not be automatically injected into the HAPSITE during a HSS run 在在 HSS 運行過程中運行過程中, 這些標準不自動注入這些標準不自動注入 HAPSITE 中中 HSS internal standards are liquid HSS 內部標準是液體內部標準是液體 Stand

23、ards must be manually injected into each sample vial using a syringe 必須用針管手動將標準注入至每個樣瓶中必須用針管手動將標準注入至每個樣瓶中Copyright 200922HSS Internal Standards HSS 內部標準內部標準 Internal standards are used as: 內部標準用作為內部標準用作為: A reference for quantitative methods 定量方法的參考定量方法的參考 An aid to estimate concentrations 幫助估算濃度幫助估

24、算濃度 4 component mix 4 成分的混合物成分的混合物 (p/n 071-748) Fluorobenzene 氟苯氟苯 (250 g/mL微克微克/毫升毫升) Chlorobenzene-d5 氯苯氯苯-d5 (250 g/mL微克微克/毫升毫升) 1,4-dichlorobenzene-d4 1,4-二氯苯二氯苯-d4 (250 g/mL微克微克/毫升毫升) Bromopentafluorobenzene 氟溴苯氟溴苯 (BPFB) (500 g/mL微克微克/毫升毫升)Copyright 200923HSS Internal Standard Addition Concen

25、tration Calculation HSS 內部標準添加物濃度計算內部標準添加物濃度計算 V1C1 = V2C2 Initial Volume x Initial Conc. = Final Volume x Final Conc. 0.010 mL x 250 m mg/mL (IS) = 20 mL x ? Final Conc. m mg/mL Final Conc. = 0.125 m mg/mL = 0.125 ppm = 125 ppbKeep the same units on both sides of the equation 公式的兩邊保持相同的單位公式的兩邊保持相同的

26、單位Internal standard concentration will vary based on sample size內部標準濃度基于樣品大小而變更內部標準濃度基于樣品大小而變更Copyright 20092424HSS 內部標準添加物濃度計算內部標準添加物濃度計算 V1C1 = V2C2 初始容積初始容積 x 初始濃度初始濃度 = 最終容積最終容積 x 最終濃度最終濃度 0.010 毫升毫升 x 250 微克微克/毫升毫升 (IS) = 20 毫升毫升 x ? 最終最終濃度濃度 微克微克/毫升毫升 最終濃度最終濃度 = 0.125 微克微克/毫升毫升 = 0.125 ppm = 1

27、25 ppb 公式的兩邊保持相同的單位公式的兩邊保持相同的單位 內部標準濃度基于樣品大小而變更內部標準濃度基于樣品大小而變更Copyright 200925HSS Internal Standards HSS 內部標準內部標準Final concentrations of various IS additions 各種各種 IS 添加物的最終濃度添加物的最終濃度SampleConfigurationAddition10 mL Water20 mL Water5 g Soil10 g SoilLoop Method (HAPSITE Smart Plus only)PPB method (HAP

28、SITE ER and Smart Plus)10 L of 250 g/mL IS0.250 g/mL(250 ppb)0.125 g/mL(125 ppb)0.500 g/g(500 ppb)0.250 g/g(250 ppb)10 L of 500 g/mL IS (BPFB)0.500 g/mL(500 ppb)0.250 g/mL(250 ppb)1.000 g/g(1 ppm)0.500 g/g(500 ppb)PPT method (HAPSITE ER and Smart Plus)1.0 L of 250 g/mL IS0.0250 g/mL(25 ppb)0.0125 g/

29、mL(12.5 ppb)0.0500 g/g(50 ppb)0.0250 g/g(25 ppb)1.0 L of 500 g/mL IS (BPFB)0.0500 g/mL(50 ppb)0.0250 g/mL(25 ppb)0.1000 g/g(100 ppb)0.0500 g/g(50 ppb)Copyright 200926HSS 內部標準內部標準各種各種 IS 添加物的最終濃度添加物的最終濃度樣品配置樣品配置添加物添加物10 毫升水毫升水20 毫升水毫升水5 克土壤克土壤10克土壤克土壤環方法環方法 (僅僅HAPSITE Smart Plus)PPB 方法方法 (HAPSITE ER

30、和和 Smart Plus)10 微升的微升的 250 微克微克/毫升毫升 IS0.250 微克微克/毫毫升升 (250 ppb)0.125微克微克/毫升毫升 (125 ppb)0.500 微克微克/克克(500 ppb)0.250微克微克/克克(250 ppb)10 微升的微升的 500微克微克/毫升毫升 IS (BPFB)0.500 微克微克/毫毫升升 (500 ppb)0.250微克微克/毫升毫升 (250 ppb)1.000微克微克/克克(1 ppm)0.500微克微克/克克(500 ppb)PPT 方法方法 (HAPSITE ER 和和 Smart Plus)1.0 微升的微升的 2

31、50微克微克/毫升毫升 IS0.0250微克微克/毫升毫升 (25 ppb)0.0125微克微克/毫升毫升 (12.5 ppb)0.0500微克微克/克克(50 ppb)0.0250微克微克/克克(25 ppb)1.0 微升的微升的 500微克微克/毫升毫升 IS (BPFB)0.0500微克微克/毫升毫升 (50 ppb)0.0250微克微克/毫升毫升 (25 ppb)0.1000微克微克/克克(100 ppb)0.0500微克微克/克克(50 ppb)Copyright 200927Preparing Blank/Samples 準備空樣準備空樣/樣品樣品 In general, HSS

32、samples are prepared as follows: 一般一般, HSS 樣品準備如下樣品準備如下: Liquids 液體液體 Fill vials full 注入樣瓶注入樣瓶 滿滿 20 mL 20 毫升毫升 Solids 固體固體 Use 10 grams 用用 10 克克 Do not block needle opening 切勿阻塞針頭的開口切勿阻塞針頭的開口 Inject 10 L internal standard for PPB (or Loop) methods 為為 PPB (或環或環)方法注入方法注入10微升內部標準微升內部標準 Inject 1 L inte

33、rnal standard for PPT methods 為為 PPT 方法注入方法注入 1 微升內部標準微升內部標準Copyright 200928Preparing Blank/Samples 準備空樣準備空樣/樣品樣品 Prepare a blank using VOC-free water and internal standard 用無用無 VOC 的水和內部標準的水和內部標準 準備空樣準備空樣 Verifies that the Headspace and the HAPSITE ER/Smart Plus are operating properly 證實頂空和證實頂空和 HA

34、PSITE ER /Smart Plus 的運行正常的運行正常 Proper gas flow 氣流量正確氣流量正確 Acceptable pressure 壓強可接受壓強可接受 Monitors carryover or contamination 監測殘留或污染監測殘留或污染Copyright 200929Preparing the Blank/Samples 準備空樣準備空樣/樣品樣品 Add 20 mL of VOC-free water into the vial 在樣瓶中添加在樣瓶中添加 20 毫升無毫升無 VOC 的水的水 Clean the 10 L glass syringe

35、 three times with methanol 用甲醇清洗用甲醇清洗 10 微升玻璃針管三次微升玻璃針管三次 Allow syringe to completely fill with methanol 針管可完全充滿甲醇針管可完全充滿甲醇 Discard excess methanol into waste vial 將過量的甲醇傾倒入廢瓶中將過量的甲醇傾倒入廢瓶中Copyright 200930 Practice proper syringe technique using methanol 用甲醇實習正確的針管技術用甲醇實習正確的針管技術 Draw the syringe plun

36、ger back to the 1 l mark 將針管柱塞拉回至將針管柱塞拉回至 1 微升標記處微升標記處 Insert the tip of the syringe needle into the methanol 將針頭的針尖插入甲醇中將針頭的針尖插入甲醇中 Be careful not to move the plunger 注意不要移動柱塞注意不要移動柱塞 Carefully draw the plunger back to the 2 l mark 小心地將柱塞拉回至小心地將柱塞拉回至 2 微升標記處微升標記處 Withdraw the syringe from the vial

37、從樣瓶中撤回針管從樣瓶中撤回針管 Be careful not to move the plunger 注意不要移動柱塞注意不要移動柱塞Preparing the Blank/Samples-Syringe Techniques準備空樣準備空樣/樣品樣品-針管技術針管技術Copyright 200931Preparing the Blank/Samples-Syringe Techniques準備空樣準備空樣/樣品樣品-針管技術針管技術Draw the plunger back until there is a 1 L plug of liquid between the 1 L and 2

38、L mark 抽回柱塞直到有抽回柱塞直到有 1 微升液體在微升液體在 1 微升與微升與 2 微升標記之間微升標記之間Plunger will be drawn back to 4 mL 柱塞被抽回至柱塞被抽回至4 毫升毫升 Inject this liquid into a waste vial 將此液體注入廢瓶中將此液體注入廢瓶中Disregard small air pockets that may appear near the plunger 不管柱塞附近是否出現小空氣泡不管柱塞附近是否出現小空氣泡Repeat this same procedure for sample prepar

39、ation 為樣品準備重復此同樣順序為樣品準備重復此同樣順序Copyright 200932Inserting Blank and Purge Vial into the HSS 將空樣和沖凈樣瓶插入將空樣和沖凈樣瓶插入 HSS 中中 Open the top lid on the Headspace 打開頂空的頂蓋打開頂空的頂蓋 Lift the needle assembly 提升針頭組件提升針頭組件 Place the standard blank into a well 將標準空樣置入槽中將標準空樣置入槽中 Place an empty vial into another well 將

40、空的樣瓶放入另一個槽中將空的樣瓶放入另一個槽中 This is the Purge Vial 這是沖凈瓶這是沖凈瓶Copyright 200933Inserting Blank and Purge Vial into the HSS 將空樣和沖凈樣瓶插入將空樣和沖凈樣瓶插入 HSS 中中 Center the needle over the septum and gently insert it into the vial for the standard blank 為標準空樣對中隔膜上的針頭為標準空樣對中隔膜上的針頭, 將它插入樣瓶將它插入樣瓶 Needle guides can be u

41、sed to help position the needle針頭導向有助于針頭定位針頭導向有助于針頭定位 Close the top lid 蓋上頂蓋蓋上頂蓋 Allow the blank to heat for 15-20 minutes 讓空樣加熱讓空樣加熱 15-20 分鐘分鐘Copyright 200934Running Analyze 運行分析運行分析Touch RUN ANALYZE 按按運行分析運行分析 Nitrogen from the headspace needle and bubble into the sample 來自頂空針頭的氮來自頂空針頭的氮, 泡狀進入泡狀進

42、入樣品樣品 This will purge the sample to release VOCs dissolved in the water 這將沖凈樣品這將沖凈樣品, 釋放溶解在水釋放溶解在水中的中的 VOCsCopyright 200935Collecting a Sample 收集樣品收集樣品 Collecting Sample For screen收集樣品用于收集樣品用于窗口窗口 The Headspace will automatically collect a sample頂空將自動收集樣品頂空將自動收集樣品 No action is required from the user

43、 用戶無需動作用戶無需動作 Sampling is Done screen 采樣完成采樣完成窗口窗口 Sample collection is complete樣品收集完成樣品收集完成 Again, no action is required from the user 同樣同樣, 用戶無需動作用戶無需動作Copyright 200936GRAPH View 圖形查看圖形查看 Compounds will be displayed on the front panel 化合物將顯示于面板上化合物將顯示于面板上 GRAPH is the default view圖形圖形是默認是默認查看查看Dis

44、plays the chromatogram during the run 顯示運行過程中的色譜圖顯示運行過程中的色譜圖 Displays:顯示顯示: Compound Name 化合物名化合物名 Retention Time 滯留時間滯留時間 TIC TIC MaxCopyright 200937CMPD ID View CMPD ID 查看查看 The data can be viewed as a list by touching CMPD ID during a run 在運行過程中在運行過程中, 按按 CMPD ID 可查看數據清單可查看數據清單 Displays:顯示顯示: Com

45、pound Name 化合物名化合物名 CAS Number CAS 數數 Retention Time 滯留時間滯留時間 Net Fit 純擬合純擬合Copyright 200938Purge Headspace 沖凈頂空沖凈頂空 A prompt will appear to purge at the end of each run 每次運行終點每次運行終點, 將出現沖凈提示將出現沖凈提示 To purge, insert the Headspace needle into the purge vial 為沖凈為沖凈, 將頂空針頭插入沖凈瓶中將頂空針頭插入沖凈瓶中 Average purg

46、e time is 2 minutes 平均沖凈時間為平均沖凈時間為 2 分鐘分鐘 Cleans residual sample from the needle 從針頭中清除殘余的樣品從針頭中清除殘余的樣品 Touch ContinueCopyright 200939Method Finished 方法完畢方法完畢 When the method and purge are complete, METHOD FINISHED will be displayed 當方法和沖凈完成時當方法和沖凈完成時, 將顯示將顯示方法完畢方法完畢字樣字樣Copyright 200940HSS Blank fro

47、m the Smart Plus 來自來自 Smart Plus 的的 HSS 空樣空樣 Chlorobenzene 氯苯氯苯-d5 (7:30)Fluorobenzene 氟苯氟苯 (2:50)1,4-Dichlorobenzene 二氯苯二氯苯 -d4 (14:30)Bromopentafluorobenzene 氟溴苯氟溴苯 (8:00 +/- 0:10)Copyright 200941HSS Blank from the ER 來自來自 ER 的的 HSS 空樣空樣Fluorobenzene 氟苯氟苯 (1:30) Chlorobenzene 氯苯氯苯-d5 (3:35)1,4-Dic

48、hlorobenzene二氯苯二氯苯 -d4 (6:45)Bromopentafluorobenzene 氟溴苯氟溴苯 (3:45 +/- 0:05)Copyright 200942Exercise: Preparing a HSS Sample 練習練習: 準備準備 HSS 樣品樣品 Use a 40 mL vial to prepare the sample用用40 毫升樣瓶準備樣品毫升樣瓶準備樣品 Add 20 mL VOC-free water 添加添加 20 毫升無毫升無 VOC 水水 Inject 1 m mL HSS Internal Standard 注入注入 1 微升微升 H

49、SS 內部標準內部標準 Inject 1 m mL Sample 注入注入 1 微升樣品微升樣品 Place the sample in the HSS and allow to equilibrate for 20 minutes 將樣品置入將樣品置入 HSS, 并讓它平衡并讓它平衡 20 分鐘分鐘 Run Method 運行方法運行方法 ER_HSS_Tri-Bed_PPB_Quant Select and use the Quant tab to view data 選用選用 Quant 標簽條查看數據標簽條查看數據Copyright 200943Worksheet: HSS Blank

50、工作表工作表: HSS 空樣空樣Copyright 200944Accessing Quantitation Reports 查訪定量報告查訪定量報告 Double-click on the ID Unknowns icon 雙擊雙擊 ID 未知物未知物 圖標圖標 The user is prompted to select the method type 提示用戶選方法類型提示用戶選方法類型 Select GC/MS 選選 GC/MS Click OK 單擊單擊 OKCopyright 200945Accessing Quantitation Reports 查訪定量報告查訪定量報告 Sel

51、ect HAPSITE 選選 HAPSITE Double-click on the desired method 雙擊所需的方法雙擊所需的方法Copyright 200946Accessing Quantitation Reports 查訪定量報告查訪定量報告 Click Browse to select the desired Data File 單擊單擊 瀏覽瀏覽 選所需的選所需的數據文件數據文件 The pathway for the data file: 數數據文件的途徑據文件的途徑: Click Start 單擊單擊 開始開始Copyright 200947Quantitation

52、 Report 定量報告定量報告Compounds Detected檢測的化合物檢測的化合物Retention Time 滯留時間滯留時間 Concentration 濃度濃度 微克微克/升升 (ppb)Copyright 200948Accessing Quantitation Reports 查訪定量報告查訪定量報告 ID Unknowns will label the peak of each chemical from the calibration library with a “T” ID 未知物未知物 將用將用 “T” 標志來自校準庫的每個化合物的峰標志來自校準庫的每個化合物的峰

53、Copyright 200949Quantitation Reports 定量報告定量報告 Use caution when using Quantitation reports 當用定量報告時當用定量報告時, 使用警示使用警示The report identifies and quantifies only the chemicals that are specifically referenced in the calibration library 報告鑒別和定量僅對校準庫中特定引用的化合物報告鑒別和定量僅對校準庫中特定引用的化合物The chromatogram must be vis

54、ually inspected for additional peaks that were not identified in the report 必須視覺檢查色譜圖是否存在報告中未鑒別的附加峰必須視覺檢查色譜圖是否存在報告中未鑒別的附加峰Copyright 200950Quantitation Reports 定量報告定量報告 ID the unidentified peak(s) using: ID 未鑒別峰用未鑒別峰用: AMDIS Search AMDIS 搜索搜索 Automatic NIST Search 自動自動 NIST 搜索搜索 Manual NIST Search 手動

55、手動 NIST 搜索搜索 Estimate the concentration 估算濃度估算濃度Copyright 20095112.5 ppb(25 if using BPFB) HSS Concentration Estimates This formula is used to estimate concentration of chemicals based on the internal standardsNOTE: This calculation is for a concentrator method using a 1 L internal standard injectio

56、nMax normalization value of nearest internal standardMax normalization value of compound of interestXApproximate Concentration ( +/- one order of magnitude)Max normalization TCE (15,900,000)Max normalization fluorobenzene (7,000,000)12.5 ppb(2.8 to 280 ppb)=X28 ppb=Copyright 20095212.5 ppb(25 if using BPFB) HSS 濃度估算濃度估算 本式用于估算基于內部標準的化合物濃度本式用于估算基于內部標準的化合物濃度注注: 本計算用于濃縮器方法本計算用于濃縮器方法, 用用 1 微升內部標準注入微升內部標準注入內部標準的內部標準的最大歸一化值