單細胞電學/ 、 倍 單細胞電學/Gentet2000Biophysical Cross2007Nature 微流 技術實現了微尺 體的控制和檢Jensen2006 Beebe2014 基于微流 技術的細胞電學/力學特性檢PNASJMEMSLabChipPNASJMMJMEMSLabChip D.HolmesandH.Morgan2009LabChip . 基于微流控的單細胞檢測平臺lock-in(dualfrequency1kHz+100kHz) cellloading

SchematicofmicrofluidicsystemforcontinuousCspecificmembraneandσcyto

Cparasitic

without

with

Cspecificmembrane=Cmembrane/Schannelcross- sm=Lcellelongation/ sm×Schannelcross-Y.Zhao,D.Y.Chen,Y.N.Luo,J.B.WangandJ.Chen,2013,Biosensorsand 基于微流控的單細胞檢測平臺 基于微流控的單細胞檢測平臺 786-Ocellline&T2cell786- 基于微流控的單細胞檢測平臺SchematicofmicrofluidicsystemforcontinuousCspecificmembraneandσcyto Distributedequivalentcircuit Y.Zhao,D.Y.Chen,Y.N.Luo,J.B.WangandJ.Chen,2013,Lab 基于微流控的單細胞檢測平臺 CspecificmembraneCMp1CMp2CMpnCspecificmembraneLcellelongationLchannelperimeter/nn1,2,3RC2RCn2RC1 1Lcellelongation/(n smSchannelcrosssection)(n2,3,4RL2RLn2RL12RLn1(n2,3,4)RleakRL1RL2RLnRLn1(n1,2,3)

RL1RC1

i1

i i2

jwCMp

RC2

* =

ni in i

1

RCnRL

Itotal* n1

Ms

MpnZeqUtotal/Itotali1*RL1i2*RL2in*RLnin1*RLn1/Itotaln1,2, 基于微流控的單細胞檢測平臺Cspecificmembraneandσcyto smasa(H1299cells,ncell=489)Cspecificmembraneandσcyto smasa(A549cells,ncell=487) 基于微流控的單細胞檢測平臺 Cspecificmembranevs. sm（H1299cells,n=489,A549,n=487 基于微流控的單細胞檢測平臺Cspecificmembranevs. 基于微流控的單細胞檢測平臺S.B.Huang,Y.Zhao,D.Y.Chen,J.B.Wang,J.Chen,M.H.Wu,2013,SensorsandActuatorsB: 21基于微流控的單細胞檢測平臺0

250350AppliednegativepressureforAppliednegativepressureforactivevalve M

R2=R2=R2=

Flow100 30μmAmplitudeAmplitude

Amptitude@1kHz

0Phase@100kHzPhase@100kHz

Phase@1kHz Phase@100kHz中國

基于微流控的單細胞檢測平臺T 驗證July13, Nov.21,10μm*10μmvs.10μm*12μm 驗證PC-3固定前 固定后的PC-3染色前S.B.Huang,Y.Zhao,...,J.Wang,J.ChenandM.H.Wu,2015, 驗證H1299vsH1299CCNYup-regulation95Dvs.95DCCNYdown-regulationY.Zhao,...,J.Wang,J.Chen,2014,Biosensorsand 驗證H1299vs.H1299CCNXup-regulationA549vsA549CypAdown-regulationY.Zhao,...,J.Wang,J.Chen,2014,Biosensorsand 應用-高遷移vs.Transwell遷移實驗95Dvs.95CY.Zhao,...,J.Wang,J.Chen,2014,Biosensorsand 應用應用MSCvs.MC-NeuronstemY.Zhao,...,J.Wang,J.Chen,PLoSone(Under

(S.Suresh,2007,Acta

(J.Guck,2005,Biophysical

(D.DiCarlo,2012, （H.Ma,2012,Labona （C.T.Lim,2009,Biomedical

(Y.Sun,2011,JournalofMicromechanicsand (C.R.Ethier,2007,CambridgeUniversity(S.Suresh,2007,Acta

EtEt 1E1E2

E2E1

1et/E2/ RawexperimentalY.Luo,...,J.W