Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemET0467Cat.No.:HY-139308CASNo.:859518-94-8分?式:C??H??F?N?分?量:441.49作?靶點:MitochondrialMetabolism作?通路:MetabolicEnzyme/Protease儲存?式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性數據體外實驗DMSO:33.33mg/mL(75.49mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制備儲備液1mM2.2651mL11.3253mL22.6506mL5mM0.4530mL2.2651mL4.5301mL10mM0.2265mL1.1325mL2.2651mL請根據產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；?旦配成溶液，請分裝保存，避免反復凍融造成的產品失效。儲備液的保存?式和期限：-80°C,6months;-20°C,1month。-80°C儲存時，請在6個?內使?，-20°C儲存時，請在1個?內使?。體內實驗請根據您的實驗動物和給藥?式選擇適當的溶解?案。以下溶解?案都請先按照InVitro?式配制澄的儲備液，再依次添加助溶劑：(為保證實驗結果的可靠性，澄的儲備液可以根據儲存條件，適當保存；體內實驗的?作液，建議您現?現配，當天使?；以下溶劑前顯?的百分?指該溶劑在您配制終溶液中的體積占?；如在配制過程中出現沉淀、析出現象，可以通過加熱和/或超聲的?式助溶)1.請依序添加每種溶劑：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(5.66mM);Clearsolution1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEBIOLOGICALACTIVITY?物活性T0467以PINK1依賴的?式激活線粒體parkin易位。T0467不誘導多巴胺能神經元中PINK1的線粒體聚集。T0467?種潛在的PINK1-Parkin信號激活化合物，可?于帕?森病及相關疾病的研究。體外研究T0467(2.5-20μM;3hours)stimulatesthemitochondrialtranslocationofGFP-Parkinover12μMinHeLa/GFP-Parkincells[1].WhenHeLa/GFP-Parkincellsaretreatedwith20μMT0467for3h,GTP-Parkinistranslocatedtothemitochondriainapproximately21%ofcells[1].T0467doesnotshowobvioustoxicityinDrosophilaatconcentrations2+responseinDrosophilalarvalmuscles[1].REFERENCES[1].KahoriShiba-Fukushima,etal.ACell-BasedHigh-ThroughputScreeningIdentifiedTwoCompoundsthatEnhancePINK1-ParkinSignaling.iScience.2020May22;23(5):101048.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792;02