1、Side Chain Assignment生物大分子波譜學原理吳季輝(H)C(CO)NH-TOCSY: Side Chain C chemical shiftsH(CC)(CO)NH-TOCSY: Side chain H chemical shiftsHCCH-COSY: adjacent C-C correlationHCCH-TOCSY: long range C-C correlation within one residueCCONH-TOCSY生物大分子波譜學原理吳季輝HCONH-TOCSY生物大分子波譜學原理吳季輝13C13C相關譜生物大分子波譜學原理吳季輝碳碳相關譜HCCH-C

2、OSY（1H-13C-13C-1H correlation spectroscopy）及HCCH-TOCSY（1H-13C-13C-1H total correlation spectroscopy）用于證認13C標記蛋白質的脂肪鏈1H及13C，信號傳遞是從1H開始，經過單鍵J偶合到13C，再通過類似同核COSY及TOCSY的機制傳遞到其他13C，最后通過單鍵J偶合回傳到1H。所以這類實驗可以看作1H同核COSY及TOCSY在13C上的推廣。除了可以獲得13C的信息，也可得到1H的信息，對于大蛋白質而言，效果比1H同核COSY及TOCSY更好，因為在1H同核COSY及TOCSY中起作用的是比較

3、小的1H同核J偶合（一般小于10Hz），而這里雖有三步傳遞，卻均是比較大的J偶合，其中JHC約140Hz，JCC約35Hz。因此這類實驗對于大蛋白質的側鏈1H，13C的證認非常重要。 13C13C相關譜生物大分子波譜學原理吳季輝生物大分子波譜學原理吳季輝生物大分子波譜學原理吳季輝生物大分子波譜學原理吳季輝生物大分子波譜學原理吳季輝生物大分子波譜學原理吳季輝生物大分子波譜學原理吳季輝生物大分子波譜學原理吳季輝生物大分子波譜學原理吳季輝7.3 13C13C相關譜生物大分子波譜學原理吳季輝7.3 13C13C相關譜生物大分子波譜學原理吳季輝7.3 13C13C相關譜生物大分子波譜學原理吳季輝7.3

4、13C13C相關譜生物大分子波譜學原理吳季輝7.3 13C13C相關譜生物大分子波譜學原理吳季輝Conventional implementation: 1H magnetization is transferred in a refocused INEPT to 13C in-phase magnetization. After C,C-TOCSY mixing, the back-transfer is achieved in an analogous manner. The incrementation of delays required for shared-time evoluti

5、on in t1 is also indicated. Note that the C 180 pulse during the t1 evolution period is not absolutely required, because 2JH,C couplings are small.Doubly sensitivity-enhanced 3D H(C)CH-TOCSY experiment with hetero-nuclear gradient echo for solvent suppression. The heteronuclear in-phase transfer is implemented with a yxz-ICOS-CT sequence. Four different combination of gradients and and of phase settings and (corresponding to echo/antiecho pathways for both indirect dimensions) have to be recorded and stored in different memory locations. Modifications required for a semi-constant-time evoluti