1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemE6-Mercaptopurine hydrateCat. No.: HY-13677ACAS No.: 6112-76-1Synonyms: Mercaptopurine hydrate; 6-MP hydrate分式: CHNOS分量: 170.19作靶點: Nucleoside Antimetabolite/Analog作通路: Cell Cycle/DNA Damage儲存式: Powder -20C 3 years4C 2 yearsIn so

2、lvent -80C 6 months-20C 1 month溶解性數據體外實驗 DMSO : 32 mg/mL (188.03 mM; Need ultrasonic and warming)Mass Solvent1 mg 5 mg 10 mg Concentration制備儲備液1 mM 5.8758 mL 29.3789 mL 58.7579 mL5 mM 1.1752 mL 5.8758 mL 11.7516 mL10 mM 0.5876 mL 2.9379 mL 5.8758 mL請根據產品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液，并請注意儲備液的保存式和期限。BIOLOGI

3、CAL ACTIVITY物活性 6-Mercaptopurine hydrate (Mercaptopurine hydrate)種嘌呤類似物， 內 性嘌呤的拮抗劑且已被泛作抗病藥物和免疫抑制藥物。IC50 & Target endogenous purines 1體外研究 6-Mercaptopurine hydrate (6-MP) induces NR4A3 transcriptional activity 1.6- to 11-fold (P 2.1/3 Master of Small Molecules 您邊的抑制劑師www.MedChemE體內研究 In the fetal tel

4、encephalons of the 6-Mercaptopurine hydrate (6-MP)-treated group, the S phase cellpopulation increases at 36 and 48 h and returns to the control level at 72 h after treatment. The G2/M phasecell population begins to increase at 24 h, peaks at 36 h, decreases at 48 h, and finally returnes to the cont

5、rollevel at 72 h. On the other hand, the sub-G1 phase cell population (apoptotic cells) begins to increase at 36h, peaks at 48 h, and then decreases at 72 h 3.PROTOCOLKinase Assay 2 L6 myotubes are treated with DMSO control or 6-Mercaptopurine hydrate (6-MP) for 24 h, with the final 3 h ofincubation

6、 including treatments in serum-free DMEM, and further incubated in the absence or presence of100 nM insulin for 60 min at 37C. Then, protein lysates (50 g) are collected and subjected to SDS.The proteins are finally quantified by densitometric analysis of scanned films using Image J software 2.MCE h

7、as not independently confirmed the accuracy of these methods. They are for reference only.Cell Assay 2 Cell viability is measured using Cell Viability Assay. L6 skeletal muscle cells are seeded in 96-well plates at adensity of 10,000 cells/well and differentiated into myotubes within 7 days. Cells a

8、re treated with differentdoses of 6-Mercaptopurine hydrate (6-MP) for 24 h before the assay. For analysis of cell viability, plates areequilibrated at room temperature for 30 min; 50 L of Cell Titer-Glo reagent is added to each well, and platesare mixed for 12 min on an orbital shaker. Luminescence

9、is quantified using a luminometer 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Around thirteen-week-old pregnant rats are used in this study. The animals are housed individually in wire-Administration 3 mesh cages in an air-conditioned room

10、(temperature, 233C; humidity, 5020%; ventilation, 10 times/hour;lighting, 12 h light to12 h dark cycle) and are given pelleted diet and water ad libitum. In the experiment,fifteen pregnant rats are injected i.p. with 50 mg/kg 6-Mercaptopurine hydrate (6-MP) on E13, and threedams each are sacrificed

11、by exsanguination from the abdominal aorta under ether anesthesia at 12, 24, 36,48, and 72 h. Fetuses are collected from each dam by Caesarean section. As controls, fifteen pregnant ratsare injected i.p. with 2.0% methylcellulose solution in distilled water at E13, and three dams are sacrificed atea

12、ch of the same time-points 3.MCE has not independently confirmed the accuracy of these methods. They are for reference only.戶使本產品發表的科研獻 J Mol Med (Berl). 2019 Jun 14.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Sahasranaman S, et al. Clinical pharmacology and pharmacogenetic

13、s of thiopurines. Eur J Clin Pharmacol. 2008 Aug;64(8):753-67.2. Liu Q, et al. 6-Mercaptopurine augments glucose transport activity in skeletal muscle cells in part via a mechanism dependent uponorphan nuclear receptor NR4A3. Am J Physiol Endocrinol Metab. 2013 Nov 1;305(9):E1081-92.2/3 Master of Small Molecules 您邊的抑制劑師www.MedChemE3. Kanemitsu H, et al. 6-Mercaptopurine (6-MP) induces cell cycle arrest and apoptosis of neural progenitor cells in the developing fetal ratbrain. Neurotoxicol Teratol. 2009 Mar-Apr;31(2):1